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Thursday, February 25, 2016

Week 2 Legionella pnuemophila Research

Okay so here is an update on what is going on with this research project.  After laboring over the research proposal I have devised a possible route of exploration.  Much of the ground work has already been established by my partners in this project.  Their goal was to find out whether or not Legionella pneumophila was present in water systems around our school.  The samples they collected underwent a series of procedures that helped isolate the DNA of the bacteria as well as positively identifying L. pneumophila using genomic sequencing.

Using similar procedures to identify the presence of L. pneumophila I would be interested in seeing whether the bacteria has higher populations during the warmer months than the cooler months. What I am proposing to study is the concentration of DNA of the bacteria and compare this to the data that already exists.  The bacteria tends to like warm environments and is especially present in hot water systems.  Since Phoenix can get so hot I feel that they may present themselves in higher numbers by also accumulating in 'cold' water systems when temperatures reach the highs we all know and love.

However there are some obstacles that must be overcome in order for this experiment to be successful.  The biggest obstacle I foresee is being able to collect enough samples while it is still relatively cool.  The warm weather we felt was caused by a heat wave and hopefully I will be able to collect relevant samples when it cools down. If it cools down. 

1 comment:

  1. Hey David! That’s awesome that you have most of the ground work already done by the team. I bet it is really helpful. It is true that the weather here will be an obstacle to find your collected data to be on a colder day. Maybe you could consider collecting data even in the mornings or nights of some of those colder days to have the maximum opportunity to get the sample on a colder temperature. However, the obstacle with that would also be that no one is here very late in the night or very early in the morning. But you’ll find a way!

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