Lab work on top of lab work (Legionella pneumophila Blog Week 10)

This week feels like we've been in the lab non-stop.  Tuesday our team finished filtrating 3 samples that we had not been able to filter last week because we ran out of autoclaved vacuum filtration glassware. Once we finished filtrating our samples we began DNA extraction from the filtered samples.  The only problem we had was that we were not using the usual DNA extraction kit that we had been using.  Instead we used a kit by Omega bio-tek. This kit used a pretty extensive protocol (roughly 30 steps) and it took our group a while to work through the extraction.  Wednesday we worked on our poster fine tuning what we think was wrong with it. I will post it here and would love to hear any feedback you guys may have on the poster.  Today also ran four Agarose Gels with the PCR product from our last PCR procedure in order to see if ASU's DNA Lab will be able to sequence these bands once we extract them.  We have our fingers crossed since we would really love to know through genomic sequencing that we found L. pneumophila in these samples.