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Thursday, April 7, 2016

Almost Through An Entire Cycle (Legionella pneumophila Blog Week 8)

I view our work in the detection of Legionella p. as a cycle.  First we must collect the samples, then we have to filter out the DNA, next is the extraction of the genetic material.  Once the genetic material is out, we amplify the DNA we want through PCR with primers specific to a gene in Legionella p. Once amplified we perform electrophoresis with a positive control and a molecular weight marker to screen for our suspect.  After all of this has been done we finally cut out the genetic material out of the gel, extract it and send it off for genomic sequencing at ASU. Then we start with gathering samples all over again.  This week we are supposed to send 4 of our samples off to ASU to get tested to see if we can absolutely identify the bacteria.  I can not wait to drop off our samples and see what we get.  Getting those results back would finally complete a cycle of research!

I'll post a picture of our group at ASU tomorrow when we go!
So here is the picture of the lab, I didn't want to take pictures of people so I tried to avoid them and this is all I could get.  
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