Hello
everyone, this is week 7 of our journey through the S-STEM internship! This week for our Legionella p. project, we had to run PCR on 10 different
samples. We ran the PCR on Tuesday and
today we ran the amplified DNA in the Agarose Gel. Unfortunately, I goofed on 4
samples that I loaded (I loaded the gel wells with 7 microliters [μL]
of material instead of 12μL). Our
mentor, Robin Cotter, advised us that it would be wise to run the PCR again on these
samples and do the electrophoresis again in order to verify our results. The reasoning is that; if the Legionella p. bacteria are present but
there was not enough genetic material in the 7μL then it may not be visible
when looking at the gels. As for the
remaining 6 samples that did run and ran well, we will be cutting out the
clearly visible bands and performing a DNA extraction process from the gels so
that we can send the samples to ASU for genomic testing. Once we know the
sequence of the nucleotides, we can compare that to the known sequence of Legionella p. and know for sure that the
bacteria were present in the samples that we collected.
That is all for now! Above is a picture of me on my birthday with
Daisy one of my group mates.
P.S. Today is my birthday!
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